全文获取类型
收费全文 | 52554篇 |
免费 | 5579篇 |
国内免费 | 2014篇 |
出版年
2023年 | 372篇 |
2022年 | 449篇 |
2021年 | 1761篇 |
2020年 | 1203篇 |
2019年 | 1437篇 |
2018年 | 1521篇 |
2017年 | 1136篇 |
2016年 | 1722篇 |
2015年 | 2492篇 |
2014年 | 2937篇 |
2013年 | 3207篇 |
2012年 | 3827篇 |
2011年 | 3629篇 |
2010年 | 2222篇 |
2009年 | 1930篇 |
2008年 | 2288篇 |
2007年 | 2131篇 |
2006年 | 1947篇 |
2005年 | 1675篇 |
2004年 | 1502篇 |
2003年 | 1349篇 |
2002年 | 1205篇 |
2001年 | 2871篇 |
2000年 | 2691篇 |
1999年 | 2084篇 |
1998年 | 797篇 |
1997年 | 766篇 |
1996年 | 720篇 |
1995年 | 681篇 |
1994年 | 584篇 |
1993年 | 433篇 |
1992年 | 1057篇 |
1991年 | 845篇 |
1990年 | 783篇 |
1989年 | 624篇 |
1988年 | 489篇 |
1987年 | 405篇 |
1986年 | 327篇 |
1985年 | 270篇 |
1984年 | 205篇 |
1983年 | 157篇 |
1982年 | 121篇 |
1981年 | 101篇 |
1980年 | 72篇 |
1979年 | 104篇 |
1978年 | 81篇 |
1977年 | 75篇 |
1975年 | 74篇 |
1974年 | 71篇 |
1973年 | 80篇 |
排序方式: 共有10000条查询结果,搜索用时 62 毫秒
31.
An eight-nucleotide sequence in the potato virus X 3' untranslated region is required for both host protein binding and viral multiplication. 总被引:3,自引:1,他引:2 下载免费PDF全文
Gel retardation and UV-cross-linking techniques were used to demonstrate that two tobacco proteins, with approximate molecular masses of 28 and 32 kDa, bind to a site within the 3' region of potato virus X (PVX) genomic RNA. The protein binding is specific, in that a 50-fold excess of unlabeled probe prevents formation of the complexes but no reduction is observed with a 2,000-fold molar excess of yeast tRNA. Complex formation is inhibited by poly(U) but is relatively unaffected by poly(A), poly(G), or poly(C-I). PVX RNA-host protein complex formation occurs in vitro at salt concentrations up to 400 mM. Deletion mapping indicates that the proteins bind within the 3' untranslated region (UTR) of PVX genomic RNA and that an 8-nucleotide U-rich sequence (5'-UAUUUUCU) is required for the binding. Deletion of the 8-nucleotide U-rich region from the 3' UTR of a sensitive PVX reporter virus that carries the luciferase gene in place of the PVX coat protein gene results in a more than 70,000-fold reduction in luciferase expression in tobacco protoplasts. RNA probes carrying the sequence GCGC in place of the central four contiguous uridines of the 8-nucleotide U-rich motif fail to bind host protein at detectable levels, and the same mutation, when introduced into the PVX reporter virus, eliminates viral multiplication. Mutations of 1 or 2 nucleotides within the same four uridines reduced both binding of host proteins and replication of reporter virus. These results indicate that the 8-nucleotide U-rich motif within the PVX 3' UTR is important for some aspect of viral multiplication and suggest that host protein binding plays a role in the process. 相似文献
32.
33.
34.
35.
Chen Chen Raymond Dagnino Jr. Charles Q. Huang James R. McCarthy Dimitri E. Grigoriadis 《Bioorganic & medicinal chemistry letters》2001,11(24):3165-3168
Cyclizations of alkylhydrazines with N-acyl-S-methylisothioureas, readily synthesized from acyl chlorides, sodium thioisocyanate, dialkylamines then methyl iodide in a one-pot reaction, gave 1-alkyl-3-dialkylamino-5-phenyltriazoles 7 as major products. The regioisomers were assigned through the use of NOE NMR experiments. While bearing a N-bis(cyclopropyl)methyl-N-propylamino group, this series of compounds shows very good binding affinity on the human CRF1 receptor. Among them, 1-methyl-3-[N-bis(cyclopropyl)methyl-N-propylamino]-5-(2,4-dichlorophenyl)-1H-[1,2,4]triazole 7a had the best binding affinity for the CRF1 receptor (Ki=9 nM). 相似文献
36.
37.
38.
Jian Xiao Dan Chen Xiu-Xian Lin Shi-Fang Peng Mei-Fang Xiao Wei-Hua Huang Yi-Cheng Wang Jing-Bo Peng Wei Zhang Dong-Sheng Ouyang Yao Chen 《PloS one》2016,11(2)
Ginsenoside compound K (CK), a rare ginsenoside originating from Panax Ginseng, has been found to possess unique pharmacological activities specifically as anti-cancers. However, the role of cytochrome P450s (CYPs) in the metabolism of CK is unclear. In this study, we screened the CYPs for the metabolism of CK in vitro using human liver microsomes (HLMs) or human recombinant CYPs. The results showed that CK inhibited the enzyme activities of CYP2C9 and CYP3A4 in the HLMs. The Km and Vmax values of CK were 84.20±21.92 μM and 0.28±0.04 nmol/mg protein/min, respectively, for the HLMs; 34.63±10.48 μM and 0.45±0.05 nmol/nmol P450/min, respectively, for CYP2C9; and 27.03±5.04 μM and 0.68±0.04 nmol/nmol P450/min, respectively, for CYP3A4. The IC50 values were 16.00 μM and 9.83 μM, and Ki values were 14.92 μM and 11.42μM for CYP2C9 and CYP3A4, respectively. Other human CYP isoforms, including CYP1A2, CYP2A6, CYP2D6, CYP2E1, and CYP2C19, showed minimal or no effect on CK metabolism. The results suggested that CK was a substrate and also inhibitors for both CYP2C9 and CYP3A4. Patients using CK in combination with therapeutic drugs that are substrates of CYP2C9 and CYP3A4 for different reasons should be careful, although the inhibiting potency of CK is much poorer than that of enzyme-specific inhibitors. 相似文献
39.
Six peripheral blood lymphoid fractions (total lymphocytes, non-T, T, Tar (autologous rosette-forming T cells/precursor), T mu (helper), and T gamma (suppressor) lymphocytes) isolated through rosetting procedures were examined for the presence of ferritin by a direct immunofluorescence technique. Although ferritin was present in all lymphoid fractions studied, a significantly higher proportion of ferritin-containing cells were detected in the T-cell fraction than in the non-T-cell fraction, (mean +/- SD = 7.9 +/- 1.6% and 5.0 +/- 1.2%, respectively). T mu- and T gamma-cell fractions showed a twofold increase in the number of ferritin-positive cells (14.1 +/- 1.4% and 15.4 +/- 2.6%, respectively), as compared with Tar (7.0 +/- 0.9%)-and total lymphocyte (6.9 +/- 1.3%)-cell fractions. These results indicate that ferritin is preferentially distributed in T mu and T gamma lymphocytes and may constitute the basis for explaining some of the roles exercised by these cells in the control of other biological systems. 相似文献
40.